Engineering of catalytic hairpin-rigidified Y-shaped DNA-functionalized nanomachine to rapidly detect mRNA.
Shufen YaoRong ZouFeng ChenHang GongChangqun CaiPublished in: Mikrochimica acta (2023)
The catalytic hairpin-rigidified Y-shaped DNA through layer-by-layer assembly has been fixed on the surface of copper sulfide nanoparticles for the detection of survivin mRNA. The distance between the CHA probes fixed on the Y-shaped DNA is significantly shortened. The results show that the fluorescence of this nanomachine reached the maximum value in 50 min (excitation wavelength at 488 nm and emission wavelength 526 nm), and its reaction rate is more than 5-fold faster than that of the free-CHA control system. In addition, the nanomachine showed high sensitivity (LOD of 3.5 pM) and high specificity for the survivin mRNA detection. Given its fast response time and excellent detection performance, we envision that the catalytic hairpin-rigidified Y-shaped DNA-functionalized nanomachine will offer potential applications in disease diagnostics and clinical applications.
Keyphrases
- single molecule
- circulating tumor
- cell free
- loop mediated isothermal amplification
- nucleic acid
- real time pcr
- label free
- photodynamic therapy
- quantum dots
- small molecule
- binding protein
- particulate matter
- circulating tumor cells
- air pollution
- energy transfer
- human health
- sensitive detection
- light emitting
- fluorescent probe