Distinct T cell subsets in adipose tissue are associated with obesity.
Martha E HaugstøylMartin CornilletKristina StrandNatalie StiglundDan SunLaurence Lawrence-ArcherIren D HjellestadErnesto SparrelidChristian BuschJøran HjelmesaethJens K HertelAndrea PonzettaGunnar MellgrenJohan FernøNiklas K BjörkströmPublished in: European journal of immunology (2022)
Adipose tissue inflammation is a driving factor for the development of obesity-associated metabolic disturbances, and a role of adipose tissue T cells in initiating the pro-inflammatory signaling is emerging. However, data on human adipose tissue T cells in obesity is limited, reflected by the lack of phenotypic markers to define tissue-resident T cell subsets. In this study, we performed a deep characterization of T cells in blood and adipose tissue depots using multicolor flow cytometry and RNA sequencing. We identified distinct subsets of T cells associated with obesity expressing the activation markers, CD26 and CCR5, and obesity-specific genes that are potentially engaged in activating pro-inflammatory pathway, including ceramide signaling, autophagy, and IL-6 signaling. These findings increase our knowledge on the heterogeneity of T cells in adipose tissue and on subsets that may play a role in obesity-related pathogenesis. This article is protected by copyright. All rights reserved.
Keyphrases
- adipose tissue
- insulin resistance
- high fat diet induced
- metabolic syndrome
- high fat diet
- weight loss
- type diabetes
- weight gain
- skeletal muscle
- oxidative stress
- healthcare
- signaling pathway
- peripheral blood
- endothelial cells
- single cell
- cell death
- gene expression
- endoplasmic reticulum stress
- deep learning
- artificial intelligence
- patient safety
- transcription factor