Combination of ultra-rapid DNA purification (PURE) and loop-mediated isothermal amplification (LAMP) for rapid detection of Trypanosoma cruzi DNA in dried blood spots.

Silvia A LonghiLady J García CasaresArturo A Muñoz-CalderónJulio Alonso-PadillaAlejandro Gabriel Schijman

Published in: PLoS neglected tropical diseases (2023)

Procedures to operate LAMP reactions from small volumes of fluid blood or DBS in FTA were standardized for LAMP detection of T. cruzi DNA. Our results encourage prospective studies in neonates born to seropositive women or oral Chagas disease outbreaks to operationally evaluate the method in the field.

Keyphrases

loop mediated isothermal amplification
circulating tumor
sensitive detection
cell free
single molecule
trypanosoma cruzi
low birth weight
nucleic acid
polycystic ovary syndrome
deep brain stimulation
circulating tumor cells
gestational age
metabolic syndrome
pregnant women
adipose tissue