Intra-Amniotic Administration of Cashew Nut ( Anacardium occidentale L.) Soluble Extract Improved Gut Functionality and Morphology In Vivo ( Gallus gallus ).
Talitha Silva MeneguelliNikolai KolbaArundhati MisraAna Paula DionísioAna Claudia Pelissari KravchychynBárbara Pereira Da SilvaHercia Stampini Duarte MartinoHelen Hermana Miranda HermsdorffElad TakoPublished in: Nutrients (2023)
Cashew nuts are rich in dietary fibers, monounsaturated fatty acids, carotenoids, tocopherols, flavonoids, catechins, amino acids, and minerals that offer benefits for health. However, the knowledge of its effect on gut health is lacking. In this way, cashew nut soluble extract (CNSE) was assessed in vivo via intra-amniotic administration in intestinal brush border membrane (BBM) morphology, functionality, and gut microbiota. Four groups were evaluated: (1) no injection (control); (2) H 2 O injection (control); (3) 10 mg/mL CNSE (1%); and (4) 50 mg/mL CNSE (5%). Results related to CNSE on duodenal morphological parameters showed higher Paneth cell numbers, goblet cell (GC) diameter in crypt and villi, depth crypt, mixed GC per villi, and villi surface area. Further, it decreased GC number and acid and neutral GC. In the gut microbiota, treatment with CNSE showed a lower abundance of Bifidobacterium , Lactobacillus , and E. coli . Further, in intestinal functionality, CNSE upregulated aminopeptidase (AP) gene expression at 5% compared to 1% CNSE. In conclusion, CNSE had beneficial effects on gut health by improving duodenal BBM functionality, as it upregulated AP gene expression, and by modifying morphological parameters ameliorating digestive and absorptive capacity. For intestinal microbiota, higher concentrations of CNSE or long-term intervention may be necessary.
Keyphrases
- gene expression
- healthcare
- public health
- mental health
- dna methylation
- single cell
- health information
- gas chromatography
- transcription factor
- cell therapy
- escherichia coli
- oxidative stress
- anti inflammatory
- ultrasound guided
- stem cells
- health promotion
- mass spectrometry
- mesenchymal stem cells
- risk assessment
- combination therapy
- bone marrow
- antibiotic resistance genes