Anti-Inflammatory Properties of Bellevalia saviczii Root Extract and Its Isolated Homoisoflavonoid (Dracol) Are Mediated by Modification on Calcium Signaling.
Monica SavioMohammed Farhad IbrahimChiara ScarlataMatteo OrgiuGiuseppe AccardoAbdullah Shakur SardarFrancesco MocciaLucia Anna StivalaGloria BrusottiPublished in: Molecules (Basel, Switzerland) (2019)
Bellevalia saviczii is a medicinal plant used as anti-rheumatic and anti-inflammatory herbal remedy in Iraqi-Kurdistan. The aim of this study was to evaluate the anti-inflammatory activity of its extract and the isolated homoisoflavonoid (Dracol) by studying the Ca2+-dependent NF-kB pathway. Nuclear translocation of p65 NF-kB subunit, as parameter of NF-kB activation, was visualized in human leukemic monocytes by immunofluorescence and Western blot analyses, after cell treatment with B. saviczii root extract or Dracol followed by Lipopolysaccharide stimulation. In parallel, Ca2+ signals responsible for NF-kB activation and levels of inflammatory cytokines were investigated. LPS-induced p65 translocation was evident in monocytes and both treatments, in particular that with Dracol, were able to counteract this activation. Intracellular Ca2+ oscillations were halted and the cytokine release reduced. These results confirm the traditional anti-inflammatory efficacy of B. saviczii and identify one of the molecules in the extract which appears to be responsible of this action.
Keyphrases
- lps induced
- anti inflammatory
- inflammatory response
- oxidative stress
- signaling pathway
- protein kinase
- endothelial cells
- rheumatoid arthritis
- stem cells
- peripheral blood
- toll like receptor
- single cell
- south africa
- induced pluripotent stem cells
- high resolution
- cell therapy
- mass spectrometry
- cell proliferation
- combination therapy
- atomic force microscopy
- high speed