Fluorescence Lifetime Imaging for quantification of targeted drug delivery in varying tumor microenvironments.

Amit Verma Vikas PandeyCatherine SherryChristopher JamesKailie MattesonJason T SmithAlena RudkouskayaXavier Intes Margarida M Barroso

Published in: bioRxiv : the preprint server for biology (2024)

Our study demonstrates that FLI is a powerful analytical tool to monitor the delivery of antibody drug tumor both in cell cultures and in vivo live systems. Especially, MFLI FRET is a unique imaging modality that can directly quantify target engagement with potential to elucidate the role of the TME in drug delivery efficacy in intact live tumor xenografts.

Keyphrases

drug delivery
cancer therapy
high resolution
single molecule
cell therapy
social media
single cell
stem cells
energy transfer
drug release
photodynamic therapy
bone marrow
climate change
fluorescence imaging