Activities of a broad-spectrum antimicrobial peptide analogue SAMP-A4-C8 and its combat against pneumonia in Staphylococcus aureus infected mice.
Rui Fang LiPu HaoKedong YinQingpeng XuShiming RenYingyuan ZhaoLan ZhangBeibei ZhangPublished in: Journal of peptide science : an official publication of the European Peptide Society (2023)
Antimicrobial peptides and their analogues have become substitutes for antibiotics in recent years. The antimicrobial peptide analogue SAMP-A4-C8 (n-octanoic-VRLLRRRI) with high antimicrobial activity was found in our lab. We speculate that it may kill pathogens by some lethal mechanism of action. In the present investigation, the microbicidal activities of SAMP-A4-C8 and its mechanism of action were investigated. The results demonstrated that SAMP-A4-C8 had lethal activities against Staphylococcus aureus and Candida albicans by cell disruption. Based on its microbicidal activities, we believe that it is worth further research for its potential as drug candidate. The results showed that SAMP-A4-C8, with low propensity to induce the resistance of S. aureus and C. albicans, could kill the persister cells of S. aureus and C. albicans, exhibited biofilm forming inhibition activity and preformed biofilm eradication ability against S. aureus and C. albicans, and displayed therapeutic potential on pneumonia in S. aureus infected mice by reducing lung inflammation. The present study provided a promising drug candidate in the war against multidrug resistance.
Keyphrases
- candida albicans
- biofilm formation
- staphylococcus aureus
- induced apoptosis
- high fat diet induced
- single cell
- molecular docking
- bone marrow
- cell cycle arrest
- methicillin resistant staphylococcus aureus
- skeletal muscle
- extracorporeal membrane oxygenation
- gram negative
- adverse drug
- mass spectrometry
- endoplasmic reticulum stress
- cell death
- intensive care unit
- signaling pathway
- antimicrobial resistance
- multidrug resistant
- electronic health record
- acute respiratory distress syndrome
- mesenchymal stem cells
- atomic force microscopy