Enzymatic Protein Immobilization on Amino-Functionalized Nanoparticles.

The immobilization of proteins on nanoparticles has received much attention in recent years. Among different approaches, enzymatic protein immobilization shows unique advantages because of its site-specific connection. OaAEP1 is a recently engineered peptide ligase which can specifically recognize an N -terminal GL residue ( NH 2 -Gly-Leu) and a C-terminal NGL amino acid residue (Asn-Gly-Leu- COOH ) and ligates them efficiently. Herein, we report OaAEP1-mediated protein immobilization on synthetic magnetic nanoparticles. Our work showed that Oa AEP1 could mediate C-terminal site-specific protein immobilization on the amino-functionalized Fe 3 O 4 nanoparticles. Our work demonstrates a new method for site-specific protein immobilization on nanoparticles.