Characterization of polyphenols in the methanol leaf extract of Bathysa gymnocarpa (Rubiaceae) by HPLC-DAD-ESI-MS/MS.
Ariel M AraujoLucas M CruzMário GomesBlanca GalloLigia M M ValenteLuis A BerruetaPublished in: Natural product research (2023)
The species Bathysa gymnocarpa K.Schum is a tree belonging to the Rubiaceae family, endemic in Brazil. So far, there are reports neither of phytochemical work nor of biological evaluation of it. The analysis by High Performance Liquid Chromatography coupled to a Diode Array Detector and a tandem Mass Spectrometer with an Electrospray Ionization source (HPLC-DAD-ESI-MS/MS) of its crude extract allowed to characterize in a complex mixture, without isolation, fourteen compounds, being two as cinnamic acid derivatives, and the others as mono-, di- and triglycosilated derivatives of the flavonols quercetin and kaempferol. These compounds are reported for the first time in Bathysa spp.
Keyphrases
- ms ms
- high performance liquid chromatography
- high resolution
- tandem mass spectrometry
- simultaneous determination
- liquid chromatography tandem mass spectrometry
- mass spectrometry
- solid phase extraction
- ultra high performance liquid chromatography
- structure activity relationship
- oxidative stress
- carbon dioxide
- biofilm formation
- emergency department
- high throughput
- magnetic resonance imaging
- staphylococcus aureus
- magnetic resonance
- image quality
- computed tomography