Simultaneous quantification of Losartan Potassium and its Active Metabolite, EXP3174 in Rabbit Plasma by Validated HPLC-PDA.

Herein we report a novel, accurate and cost-effective validated analytical method for quantification of losartan potassium and its active metabolite, EXP 3174 in rabbit plasma by reversed phase-high performance liquid chromatography. Valsartan was used as an internal standard. The method was validated as per ICH guidelines. The analytes were extracted in rabbit plasma using liquid-liquid extraction technique and analyzed at 247 nm after separation through a reverse phase C 18 column. The isocratic mobile phase used is a mixture of acetonitrile, water and glacial acetic acid in the ratio of 60:40:1v/v/v maintained at pH 3.4. All calibration curves showed a good linear relationship (r>0.995) within test range. Precision was evaluated by intra and inter-day tests with RSDs <1.91%, accuracy validation recovery 86.20-101.11%. Based on our results, the developed method features good quantification parameters and can serve as effective quality control method for standardization of drugs.