2D in Seconds: Coupling of Chip-HPLC with Ion Mobility Spectrometry.
Sebastian K PiendlChristian-Robert RaddatzNora T HartnerChristian ThobenRico WariasStefan ZimmermannDetlev BelderPublished in: Analytical chemistry (2019)
The online hyphenation of chip-based high-performance liquid chromatography (chip-HPLC) with ion mobility spectrometry (IMS) via fully integrated electrospray emitters is introduced. A custom-built drift tube IMS with shifted potentials was developed in order to keep the IMS orifice electrically grounded, allowing for a robust coupling with chip-HPLC. Proof-of-concept studies with the newly developed analytical setup revealed the suitability of IMS as a promising and powerful detection concept for chip-based separation techniques. Comparison of IMS with fluorescence detection and electrospray ionization-mass spectrometry (ESI-MS) allowed a more detailed characterization of the IMS as a new detection method for chip-HPLC. Moreover, the analysis of a mixture consisting of three isobaric antidepressants demonstrated the performance of chip-HPLC/IMS as a miniaturized two-dimensional separation technique.
Keyphrases
- high performance liquid chromatography
- mass spectrometry
- ms ms
- liquid chromatography
- solid phase extraction
- simultaneous determination
- tandem mass spectrometry
- circulating tumor cells
- high throughput
- gas chromatography
- high resolution
- capillary electrophoresis
- loop mediated isothermal amplification
- social media
- healthcare
- real time pcr
- single cell
- multiple sclerosis
- major depressive disorder
- quantum dots
- room temperature
- electron transfer
- bipolar disorder
- sensitive detection