Automated Buildup of Biomimetic Films in Cell Culture Microplates for High-Throughput Screening of Cellular Behaviors.
Paul MachillotCatarina QuintalFabien DalonneauLoic HermantPauline MonnotKelsey MatthewsVincent FitzpatrickJie LiuIsabelle Pignot-PaintrandCatherine PicartPublished in: Advanced materials (Deerfield Beach, Fla.) (2018)
An automatic method is established for layer-by-layer (LbL) assembly of biomimetic coatings in cell culture microplates using a commercial liquid-handling robot. Highly homogeneous thin films are formed at the bottom of each microwell. The LbL film-coated microplates are compatible with common cellular assays, using microplate readers and automated microscopes. Cellular adhesion is screened on crosslinked and peptide-functionalized LbL films and stem cell differentiation in response to increasing doses of bone morphogenetic proteins (2, 4, 7, 9). This method paves the way for future applications of LbL films in cell-based assays for regenerative medicine and high-throughput drug screening.
Keyphrases
- high throughput
- room temperature
- single cell
- deep learning
- machine learning
- ionic liquid
- bone mineral density
- stem cells
- emergency department
- biofilm formation
- bone marrow
- tissue engineering
- adverse drug
- high resolution
- body composition
- bone regeneration
- bone loss
- cell migration
- tandem mass spectrometry
- candida albicans