Identification of Metal-Binding Peptides and Their Conjugation onto Nanoparticles of Superparamagnetic Iron Oxides and Liposomes.
Tomonobu KodamaAkifumi YoshiharaIsha GoelMasaki SekinoAkihiro KuwahataAtsushi YoshimoriYuichi MurayamaKazuhiko IshiharaKristina N EkdahlBo NilssonYuji TeramuraPublished in: ACS applied materials & interfaces (2020)
Metallic materials are used for clinical medical devices such as vascular stents and coils to treat both ischemic and hemorrhagic vascular diseases. An antiplatelet drug is required to avoid thromboembolic complication until metallic surface is covered with a neo-endothelial cell layer. It is important to identify endothelial cell coverage on the metallic surface. However, it is difficult since there are no selective ligands. Here, we used the phage display method to identify peptide ligands that had high affinity for the metallic surface of Ni-Ti stents, Pt-W coils, and Co-Cr stents. The binding assay using fluorescence labeling revealed that several synthetic peptides could bind onto those surfaces. We also chose some oligopeptides for the conjugation onto superparamagnetic iron oxide (SPIO) nanoparticles and liposome-encapsulating SPIO nanoparticles and studied their ability to bind to the stent and coils. By SEM and fluorophotometry, we found that those modified SPIOs and liposomes were selectively bound onto those surfaces. In addition, both treated stents and coils could be detected by magnetic resonance imaging due to the magnetic artifact through the SPIOs and liposomes that were immobilized onto the surface. Thus, we identified metal-binding peptides which may enable to stop antiplatelet therapy after vascular stenting or coiling.
Keyphrases
- iron oxide
- antiplatelet therapy
- drug delivery
- magnetic resonance imaging
- endothelial cells
- acute coronary syndrome
- percutaneous coronary intervention
- computed tomography
- binding protein
- amino acid
- high throughput
- dna binding
- biofilm formation
- pseudomonas aeruginosa
- emergency department
- escherichia coli
- atrial fibrillation
- staphylococcus aureus
- high glucose
- single molecule
- transcription factor
- brain injury
- mass spectrometry
- metal organic framework