As a new type of nanomaterial, DNA-templated silver nanoclusters (DNA-AgNCs) have been widely studied because of their fluorescence and antibacterial properties. In this study, we combined the DNA-AgNCs with aptamers of bacteria to achieve a novel approach for the visual detection and effective elimination of bacteria. The aptamers of Staphylococcus aureus (S. aureus) were linked to G-rich sequences to achieve fluorescence enhancement when approaching the DNA-AgNCs. The capture of aptamers not only realized the visual monitoring of bacteria but also promoted the antibacterial effects. Additionally, a fluorescent nanofilm with excellent selectivity and antibacterial activity in the detection and elimination of S. aureus was developed based on the DNA-AgNCs. These aptamer-functionalized DNA-AgNCs show significant potential for many applications in food packaging and biomedical engineering.
Keyphrases
- circulating tumor
- single molecule
- cell free
- nucleic acid
- label free
- gold nanoparticles
- staphylococcus aureus
- silver nanoparticles
- quantum dots
- sensitive detection
- living cells
- risk assessment
- escherichia coli
- loop mediated isothermal amplification
- real time pcr
- molecularly imprinted
- high resolution
- energy transfer
- candida albicans
- magnetic nanoparticles