Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway.
Vinoth Kumar RethineswaranYeon-Ju KimWoong Bi JangSeung Taek JiSonghwa KangDa Yeon KimJi Hye ParkLe Thi Hong VanLy Thanh Truong GiangJong Seong HaJisoo YunDong Hyung LeeSun-Nyoung YuSul-Gi ParkSoon-Cheol AhnSang-Mo KwonPublished in: Marine drugs (2019)
The purpose of the present study is to improve the endothelial progenitor cells (EPC) activation, proliferation, and angiogenesis using enzyme-aided extraction of fucoidan by amyloglucosidase (EAEF-AMG). Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation by reducing the reactive oxygen species (ROS) and decreasing apoptosis. Notably, EAEF-AMG treated EPCs repressed the colocalization of TSC2/LAMP1 and promoted perinuclear localization of mTOR/LAMP1 and mTOR/Rheb. Moreover, EAEF-AMG enhanced EPC functionalities, including tube formation, cell migration, and wound healing via regulation of AKT/Rheb signaling. Our data provided cell priming protocols to enhance therapeutic applications of EPCs using bioactive compounds for the treatment of CVD.
Keyphrases
- signaling pathway
- reactive oxygen species
- cell migration
- cell proliferation
- pi k akt
- wound healing
- induced apoptosis
- cell death
- epithelial mesenchymal transition
- endothelial cells
- dna damage
- cell cycle arrest
- single cell
- endoplasmic reticulum stress
- loop mediated isothermal amplification
- stem cells
- electronic health record
- cell therapy
- bone marrow
- machine learning
- vascular endothelial growth factor
- artificial intelligence