Ultrasensitive and simultaneous determination of RNA modified nucleotides by sheathless interfaced capillary electrophoresis-tandem mass spectrometry.
Yue YuSi-Hao ZhuFang YuanXiao-Hui ZhangYan-Ye LuYing-Lin ZhouXin-Xiang ZhangPublished in: Chemical communications (Cambridge, England) (2019)
A label-free ultrasensitive determination of eight RNA modified nucleotides simultaneously was first established based on a sheathless capillary electrophoresis-tandem mass spectrometry system. This system performed well using only 500 pg-5 ng practical RNA samples, and a downward trend of most target nucleotides in HCT 116 cells was observed with the increase of nickel concentration.
Keyphrases
- capillary electrophoresis
- tandem mass spectrometry
- simultaneous determination
- mass spectrometry
- liquid chromatography
- label free
- ultra high performance liquid chromatography
- solid phase extraction
- high performance liquid chromatography
- molecularly imprinted
- gas chromatography
- liquid chromatography tandem mass spectrometry
- high resolution mass spectrometry
- high resolution
- cell cycle arrest
- gold nanoparticles
- induced apoptosis
- quantum dots
- cell death
- endoplasmic reticulum stress