A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing.
Biraja C DashOcean SetiaJolanta GoreckaHassan PeyvandiKaiti DuanLara LopesJames NieFrancois BerthiaumeAlan DardikHenry C HsiaPublished in: Cells (2020)
The application of human-induced pluripotent stem cells (hiPSCs) to generate vascular smooth muscle cells (hiPSC-VSMCs) in abundance is a promising strategy for vascular regeneration. While hiPSC-VSMCs have already been utilized for tissue-engineered vascular grafts and disease modeling, there is a lack of investigations exploring their therapeutic secretory factors. The objective of this manuscript was to understand how the biophysical property of a collagen-based scaffold dictates changes in the secretory function of hiPSC-VSMCs while developing hiPSC-VSMC-based therapy for durable regenerative wound healing. We investigated the effect of collagen fibrillar density (CFD) on hiPSC-VSMC's paracrine secretion and cytokines via the construction of varying density of collagen scaffolds. Our study demonstrated that CFD is a key scaffold property that modulates the secretory function of hiPSC-VSMCs. This study lays the foundation for developing collagen-based scaffold materials for the delivery of hiPSC-VSMCs to promote regenerative healing through guiding paracrine signaling pathways.
Keyphrases
- vascular smooth muscle cells
- tissue engineering
- wound healing
- angiotensin ii
- induced pluripotent stem cells
- stem cells
- endothelial cells
- signaling pathway
- oxidative stress
- mass spectrometry
- cell proliferation
- bone marrow
- pi k akt
- cell therapy
- antibiotic resistance genes
- epithelial mesenchymal transition
- endoplasmic reticulum stress
- single molecule