Optimization of processing female genital tissue samples for lymphocyte analysis by flow cytometry.

Kevin A StonerMay A BeamerHilary A AvoliaLeslie A MeynSharon L HillierSharon L Achilles

Published in: American journal of reproductive immunology (New York, N.Y. : 1989) (2020)

Although tissue digestion with collagenase D was sufficient to recover and analyze cells from endometrial biopsy specimens, cervical biopsy specimens required a limited exposure to collagenase B at 1 mg/mL to optimize cell yield and viability for cytometric analysis. PBMCs can be used as a model to assess the impact of tissue processing on co-receptor expression and to optimize methods prior to study implementation.

Keyphrases

flow cytometry
fine needle aspiration
healthcare
primary care
ultrasound guided
stem cells
single cell
cell therapy
peripheral blood
mesenchymal stem cells