Rapid and Live-Cell Detection of Senescence in Mesenchymal Stem Cells by Micro Magnetic Resonance Relaxometry.
Smitha Surendran ThamarathChing Ann TeeShu Hui NeoDahou YangRashidah OthmanLaurie A BoyerJongyoon HanPublished in: Stem cells translational medicine (2023)
Detection of cellular senescence is important quality analytics of cell therapy products, including mesenchymal stromal cells (MSCs). However, its detection is critically limited by the lack of specific markers and the destructive assays used to read out these markers. Here, we establish a rapid, live-cell assay for detecting senescent cells in heterogeneous mesenchymal stromal cell (MSC) cultures. We report that the T2 relaxation time measured by microscale Magnetic Resonance Relaxometry, which is related to intracellular iron accumulation, correlates strongly with senescence markers in MSC cultures under diverse conditions, including different passages and donors, size-sorted MSCs by inertial spiral microfluidic device, and drug-induced senescence. In addition, the live-cell and non-destructive method presented here has general applicability to other cells and tissues and can critically advance our understanding of cellular senescence.
Keyphrases
- cell therapy
- mesenchymal stem cells
- loop mediated isothermal amplification
- magnetic resonance
- bone marrow
- dna damage
- endothelial cells
- drug induced
- induced apoptosis
- umbilical cord
- stress induced
- high throughput
- liver injury
- label free
- cell cycle arrest
- stem cells
- single cell
- real time pcr
- gene expression
- sensitive detection
- endoplasmic reticulum stress
- single molecule
- magnetic resonance imaging
- cell death
- oxidative stress
- big data
- computed tomography
- circulating tumor cells
- cell proliferation
- signaling pathway
- artificial intelligence
- quantum dots
- iron deficiency