Ratiometric fluorescence imaging of lysosomal NO in living cells and mice brains with Alzheimer's disease.
Rui HuangZiwei ZhangZhen ShiYumeng YangJunyong SunFeng GaoPublished in: Chemical communications (Cambridge, England) (2024)
We report an integrated ratiometric lysosomal nitric oxide (NO) nanoprobe based on engineered semiconducting polymer dots (Pdots), LyNO-Pdots, which consist of a newly designed NO-responsive dye, a fluorescent conjugated polymer and two functional polymers. The developed probe LyNO-Pdots exhibit high specificity and stability, good photostability and favorable blood-brain barrier (BBB) penetration ability. The LyNO-Pdots are successfully applied to ratiometric imaging of lysosomal NO variations in brain-derived endothelial cells, brain tissues and mice brains with Alzheimer's disease (AD). The results demonstrate that the NO content in the brains of AD mice is considerably higher than that in normal mice.
Keyphrases
- living cells
- fluorescent probe
- blood brain barrier
- single molecule
- fluorescence imaging
- high fat diet induced
- nitric oxide
- quantum dots
- endothelial cells
- cerebral ischemia
- high resolution
- gene expression
- cognitive decline
- white matter
- adipose tissue
- type diabetes
- insulin resistance
- nitric oxide synthase
- energy transfer
- highly efficient
- metabolic syndrome
- high glucose
- functional connectivity