Liquid NanoBiosensors Enable One-Pot Electrochemical Detection of Bacteria in Complex Matrices.
Sara M ImaniEnas OsmanFatemeh BakhshandehShuwen QianSadman SakibMichael MacDonaldMark GaskinIgor ZhitomirskyDeborah YamamuraYingfu LiTohid F DidarLeyla SoleymaniPublished in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2023)
There is a need for point-of-care bacterial sensing and identification technologies that are rapid and simple to operate. Technologies that do not rely on growth cultures, nucleic acid amplification, step-wise reagent addition, and complex sample processing are the key for meeting this need. Herein, multiple materials technologies are integrated for overcoming the obstacles in creating rapid and one-pot bacterial sensing platforms. Liquid-infused nanoelectrodes are developed for reducing nonspecific binding on the transducer surface; bacterium-specific RNA-cleaving DNAzymes are used for bacterial identification; and redox DNA barcodes embedded into DNAzymes are used for binding-induced electrochemical signal transduction. The resultant single-step and one-pot assay demonstrates a limit-of-detection of 10 2 CFU mL -1 , with high specificity in identifying Escherichia coli amongst other Gram positive and negative bacteria including Klebsiella pneumoniae, Staphylococcus aureus, and Bacillus subtilis. Additionally, this assay is evaluated for analyzing 31 clinically obtained urine samples, demonstrating a clinical sensitivity of 100% and specify of 100%. When challenging this assay with nine clinical blood cultures, E. coli-positive and E. coli-negative samples can be distinguished with a probability of p < 0.001.
Keyphrases
- nucleic acid
- escherichia coli
- klebsiella pneumoniae
- loop mediated isothermal amplification
- label free
- ionic liquid
- bacillus subtilis
- high throughput
- staphylococcus aureus
- multidrug resistant
- gold nanoparticles
- biofilm formation
- gram negative
- diabetic rats
- sensitive detection
- single molecule
- binding protein
- molecularly imprinted
- bioinformatics analysis
- real time pcr
- drug induced
- circulating tumor
- endothelial cells
- oxidative stress
- mass spectrometry