Microvirga terrae sp. nov., Isolated from Soil.

A Gram-stain-negative and strictly aerobic bacterium, strain R24 T , was isolated from soil in South Korea. Cells were non-motile short rods showing catalase- and oxidase-positive activities. Growth was observed at 15-40 °C (optimum, 25-30 °C) and pH 6.0-10.0 (optimum, 8.0-9.0), and in the presence of 0-3.0% NaCl (optimum, 0%). Strain R24 T contained ubiquinone-10 as the sole respiratory quinone, C 16:0 , C 18:0 , and summed feature 8 (comprising C 18:1 ω7c and/or C 18:1 ω6c) as the major fatty acids, and phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylcholine as the major polar lipids. The DNA G+C content calculated from the whole genome sequence was 64.4%. Strain R24 T was most closely related to Microvirga aerilata 5420S-16 T with a 98.6% 16S rRNA gene sequence similarity. Average nucleotide identity and digital DNA-DNA hybridization values between strain R24 T and all Microvirga species were less than 82.5 and 23.8%, respectively. Phylogenetic analyses based on the 16S rRNA gene and whole genome sequences revealed that strain R24 T formed a phyletic lineage within the genus Microvirga. Based on its phenotypic, chemotaxonomic, and molecular characteristics, strain R24 T represents a novel species of the genus Microvirga, for which the name Microvirga terrae sp. nov. is proposed. The type strain is R24 T (= KACC 21784 T  = JCM 34259 T ).