Disposable impedance sensors based on novel hybrid MoS2 nanosheets and microparticles to detect Escherichia Coli DNA.
Tien Ngoc Phuc NguyenSon Hai NguyenMai Thi TranPublished in: PloS one (2024)
The rapid and accurate detection of pathogenic bacteria is essential for food safety and public health. Conventional detection techniques, such as nucleic acid sequence-based amplification and polymerase chain reaction, are time-consuming and require specialized equipment and trained personnel. Here, we present quick, disposable impedance sensors based on the novel hybrid MoS2 nanomaterial for detecting Escherichia coli DNA. Our results indicate that the proposed sensors operate linearly between 10- 20 and 10-15 M concentrations, achieving an impressive detection limit of 10-20 M with the highest sensitivity observed at a 0.325 nM probe concentration sensor. Furthermore, the electrochemical impedance spectroscopy biosensors exhibited potential selectivity for Escherichia coli DNA over Bacillus subtilis and Vibrio proteolyticus DNA sequences. The findings offer a promising avenue for efficient and precise DNA detection, with potential implications for broader biotechnology and medical diagnostics applications.
Keyphrases
- nucleic acid
- escherichia coli
- circulating tumor
- loop mediated isothermal amplification
- label free
- single molecule
- cell free
- public health
- quantum dots
- real time pcr
- healthcare
- bacillus subtilis
- high resolution
- biofilm formation
- gold nanoparticles
- palliative care
- human health
- sensitive detection
- circulating tumor cells
- reduced graphene oxide
- highly efficient
- klebsiella pneumoniae
- mass spectrometry
- body composition
- simultaneous determination