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Identification and characterization of reaction products of 5-hydroxytryptamine with methylglyoxal and glyoxal by liquid chromatography/tandem mass spectrometry.

L Sai SachinR Nagarjuna CharyP PavankumarPrabhakar Sripadi
Published in: Rapid communications in mass spectrometry : RCM (2019)
5-HT reacts with one or two MGO/GO to form a set of reaction products. The reaction between 5-HT and MGO or GO was faster at higher concentrations of MGO/GO (<10 min), and the same products were found even at physiological concentrations (<48 h). The LC/MS/MS (SRM) method can be used to screen the reaction products when present at low levels.
Keyphrases
  • liquid chromatography tandem mass spectrometry
  • simultaneous determination
  • high throughput
  • solid phase extraction
  • single cell
  • liquid chromatography