Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts.
Anggy Lusanna Gutiérrez-OrtizVeronica VidaMatjaž PeterkaJasmina TušarFederico BertiLuciano NavariniCristina ForzatoPublished in: Sensors (Basel, Switzerland) (2022)
Green coffee beans are particularly rich in chlorogenic acids (CGAs), and their identification and quantification are usually performed by HPLC, coupled with mass spectrometry (LC-MS). Although there are a few examples of molecularly imprinted polymers (MIPs) for chlorogenic acid (5-CQA) recognition present in the literature, none of them are based on optical fluorescence, which is very interesting given its great sensitivity. In the present manuscript, fluorescent polymeric imprinted nanoparticles were synthetized following the non-covalent approach using hydrogenated 5- O -caffeoylquinic acid (H-5-CQA) as the template. The capability of the polymer to bind 5-CQA was evaluated by HPLC and fluorescence. A real sample of coffee extract was also analyzed to verify the selectivity of the polymer. Polymer fMIP01, containing 4-vinylpyridine and a naphtalimide derivative as monomers, showed a good response to the fluorescence quenching in the range 39 μM-80 mM. In the real sample, fMIP01 was able to selectively bind 5-CQA, while caffeine was not recognized. To demonstrate this, there is a promising system that can be exploited in the design of an optical sensor for 5-CQA detection. Polymer fMIP01 was immobilized by physical entrapment on a functionalized glass surface, showing a quenching of fluorescence with an increase of the CGA concentration between 156 μM and 40 mM.
Keyphrases
- molecularly imprinted
- solid phase extraction
- energy transfer
- quantum dots
- high performance liquid chromatography
- mass spectrometry
- single molecule
- liquid chromatography
- simultaneous determination
- ms ms
- high resolution
- tandem mass spectrometry
- gas chromatography
- living cells
- label free
- physical activity
- mental health
- capillary electrophoresis
- sensitive detection
- ionic liquid
- loop mediated isothermal amplification