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Deamination-triggered exponential signal amplification for chemiluminescent detection of cytosine deaminase at the single-cell level.

Wen-Jing LiuHai-Juan LiXiaoran ZouQian LiuFei MaShuangshuang Zhang
Published in: Chemical communications (Cambridge, England) (2023)
We construct a sensitive chemiluminescent biosensor for sensitive detection of cytosine deaminase APOBEC3A based on deamination-triggered exponential signal amplification. This biosensor displays good specificity and high sensitivity, and it can screen APOBEC3A inhibitors and measure endogenous APOBEC3A at the single-cell level, with prospective applications in disease diagnostics and therapy.
Keyphrases
  • sensitive detection
  • single cell
  • loop mediated isothermal amplification
  • label free
  • high throughput
  • rna seq
  • quantum dots
  • nucleic acid
  • gold nanoparticles
  • stem cells
  • mesenchymal stem cells
  • smoking cessation