Whole-genome sequencing establishes persistence of biofilm-associated Pseudomonas aeruginosa detected from microbiological surveillance of gastrointestinal endoscopes.
G S E TanG J M ChiaN M ThevasagayamS Q D LoyS R S PrakkiZ Q LimJ Y ChuaJ W Z ChiaK MarimuthuS VasooO T NgB F PohB S P AngPublished in: The Journal of hospital infection (2024)
The WGS findings helped to deprioritize common source contamination and supported the hypothesis of biofilm build-up within endoscopes, leading to repeatedly positive MS cultures that were genomically linked. This was possibly related to incomplete reprocessing of the damaged air/water valves, resulting in biofilm build-up. All faulty valves were changed and subsequently cleaned separately with ultrasonic cleaning followed by sterilization which resolved this incident.
Keyphrases
- pseudomonas aeruginosa
- biofilm formation
- cystic fibrosis
- staphylococcus aureus
- candida albicans
- acinetobacter baumannii
- aortic valve
- aortic valve replacement
- multiple sclerosis
- cardiovascular disease
- mass spectrometry
- public health
- ms ms
- drinking water
- health risk
- escherichia coli
- heart failure
- transcatheter aortic valve replacement
- multidrug resistant
- drug resistant
- ejection fraction