Development of Genomic Resources in Mexican <i>Bursera</i> (Section: <i>Bullockia:</i> Burseraceae): Genome Assembly, Annotation, and Marker Discovery for Three Copal Species.

<i>Bursera</i> comprises ~100 tropical shrub and tree species, with the center of the species diversification in Mexico. The genomic resources developed for the genus are scarce, and this has limited the study of the gene flow, local adaptation, and hybridization dynamics. In this study, based on ~155 million Illumina paired-end reads per species, we performed a de novo genome assembly and annotation of three <i>Bursera</i> species of the <i>Bullockia</i> section: <i>Bursera bipinnata, Bursera cuneata,</i> and <i>Bursera palmeri</i>. The total lengths of the genome assemblies were 253, 237, and 229 Mb for <i>B. cuneata</i>, <i>B. palmeri</i>, and <i>B. bipinnata</i>, respectively. The assembly of <i>B. palmeri</i> retrieved the most complete and single-copy BUSCOs (87.3%) relative to <i>B. cuneata</i> (86.5%) and <i>B. bipinnata</i> (76.6%). The ab initio gene prediction recognized between 21,000 and 32,000 protein-coding genes. Other genomic features, such as simple sequence repeats (SSRs), were also detected. Using the de novo genome assemblies as a reference, we identified single-nucleotide polymorphisms (SNPs) for a set of 43 <i>Bursera</i> individuals. Moreover, we mapped the filtered reads of each <i>Bursera</i> species against the chloroplast genomes of five Burseraceae species, obtaining consensus sequences ranging from 156 to 160 kb in length. Our work contributes to the generation of genomic resources for an important but understudied genus of tropical-dry-forest species.