DNAzyme-powered nucleic acid release from solid supports.

Ting CaoYongcheng WangYe TaoLexiang ZhangYing-Lin ZhouXin-Xiang ZhangJohn A HeymanDavid A Weitz

Published in: Chemical communications (Cambridge, England) (2019)

Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

Keyphrases

nucleic acid
living cells
label free
fluorescent probe
dna binding
single molecule
single cell
cell therapy
transcription factor
high throughput
circulating tumor
bone marrow
wound healing
hyaluronic acid