A hydrophilic magnetic MOF for the consecutive enrichment of exosomes and exosomal phosphopeptides.

Published in: Chemical communications (Cambridge, England) (2021)

Hydrophilic Fe3O4@PDA@UIO-66-NH2 is employed to consecutively enrich urinary exosomes and exosomal phosphopeptides through the strong bond between Zr and phosphate groups. The recovery of exosomes reached 95% and 707 phosphopeptides were identified from 255 phosphoproteins via LC-MS/MS. This work will make a great contribution to further functional analysis of exosomes.

Keyphrases

mesenchymal stem cells
stem cells
liquid chromatography
metal organic framework
bone marrow
mass spectrometry
computed tomography
room temperature
high resolution
solid phase extraction
pet ct