Expression Profiles of Long Noncoding RNA and mRNA in Epicardial Adipose Tissue in Patients with Heart Failure.
Meili ZhengLei ZhaoXin-Chun YangPublished in: BioMed research international (2019)
The expression profile of long noncoding RNA (lncRNA) in human epicardial adipose tissue (EAT) has not been widely studied. In the present study, we performed RNA sequencing to analyze the expression profiles of lncRNA and mRNA in EAT in coronary artery disease (CAD) patients with and without heart failure (HF). Our results showed RNA sequencing disclosed 35673 mRNA and 11087 lncRNA corresponding to 15554 genes in EAT in total, while 30 differentially expressed lncRNAs (17 upregulated and 13 downregulated) and 278 differentially expressed mRNAs (129 upregulated and 149 downregulated) were discriminated between CAD patients with and without HF (P<0.05; fold change>2); lncRNA ENST00000610659 drew specific attention for it was the top upregulated lncRNA with highest fold change and corresponded to UNC93B1 gene, which was proved to be related to HF and encoded UNC93B1 protein regulating toll-like receptor signaling, and both of them significantly increased in HF patients in qRT-PCR validation; the top significant upregulated enriched GO terms and KEGG pathway analysis were regulation of lymphocyte activation (GO:0051249) and T cell receptor signaling pathway (hsa04660), respectively. The current findings support the fact that EAT lncRNAs are involved in the inflammatory response leading to the development of HF.
Keyphrases
- long noncoding rna
- coronary artery disease
- toll like receptor
- adipose tissue
- inflammatory response
- acute heart failure
- heart failure
- binding protein
- signaling pathway
- genome wide identification
- end stage renal disease
- genome wide analysis
- single cell
- insulin resistance
- nuclear factor
- long non coding rna
- genome wide
- ejection fraction
- percutaneous coronary intervention
- chronic kidney disease
- working memory
- cardiovascular events
- high fat diet
- immune response
- coronary artery bypass grafting
- lipopolysaccharide induced
- atrial fibrillation
- lps induced
- transcription factor
- pi k akt
- left ventricular
- peritoneal dialysis
- oxidative stress
- type diabetes
- copy number
- small molecule
- amino acid
- prognostic factors
- protein protein
- network analysis