Effect of Fe on inorganic polyphosphate level in autotrophic and heterotrophic cells of Rhodospirillum rubrum.

Inorganic polyphosphate is involved in metal homeostasis in microorganisms. The aim of the study was to reveal differences in polyphosphate metabolism of Rhodospirillum rubrum under autotrophic and heterotrophic cultivation in the presence of Fe (2.3 mg Fe3+ L-1) and without Fe (traces). Heterotrophic conditions without Fe resulted in cell lysis and low biomass yield. High polyphosphate content and low exopolyphosphatase activity were observed in the cells cultivated autotrophically in the presence of Fe. The cells grown heterotrophically in the presence of Fe contained more phosphate and low-molecular polyphosphate; on the contrary, the content of the high molecular polyphosphate decreased in parallel with the increase in exopolyphosphatase activity. The possible involvement of Pi and polyphosphate to the formation of Fe-containing inclusions is discussed.