Determination of N-acylhomoserine lactones of Pseudomonas aeruginosa in clinical samples from dogs with otitis externa.
Darja KušarKarin ŠrimpfPetra IsakovićLina KalšekJavid HosseiniIrena ZdovcTina KotnikModest VenguštGabrijela Tavčar-KalcherPublished in: BMC veterinary research (2016)
Our results indicate that liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is superior in detecting AHLs compared to other chromatographic techniques. This is the first report on determination of AHLs in the clinical samples of veterinary importance. The analytical procedure described in this paper is capable of supporting novel antimicrobial strategies, which target quorum sensing.
Keyphrases
- liquid chromatography
- tandem mass spectrometry
- solid phase extraction
- simultaneous determination
- ultra high performance liquid chromatography
- mass spectrometry
- high performance liquid chromatography
- high resolution mass spectrometry
- pseudomonas aeruginosa
- gas chromatography
- molecularly imprinted
- high resolution
- staphylococcus aureus
- minimally invasive
- escherichia coli
- acinetobacter baumannii
- candida albicans