DNA Nanotechnology for Investigating Mechanical Signaling in the Immune System.
Yuesong HuYuxin DuanKhalid SalaitaPublished in: Angewandte Chemie (International ed. in English) (2023)
Immune recognition occurs at specialized cell-cell junctions when immune cells and target cells physically touch. In this junction, groups of receptor-ligand complexes assemble and experience molecular forces that are ultimately generated by the cellular cytoskeleton. These forces are in the range of piconewton (pN) but play crucial roles in immune cell activation and subsequent effector responses. In this minireview, we will review the development of DNA based molecular tension sensors and their applications in mapping and quantifying mechanical forces experienced by immunoreceptors including T-cell receptor (TCR), Lymphocyte function-associated antigen (LFA-1), and the B-cell receptor (BCR) among others. In addition, we will highlight the use of DNA as a mechanical gate to manipulate mechanotransduction and decipher how mechanical forces regulate antigen discrimination and receptor signaling.
Keyphrases
- single molecule
- circulating tumor
- cell free
- single cell
- cell therapy
- induced apoptosis
- regulatory t cells
- palliative care
- tyrosine kinase
- mass spectrometry
- oxidative stress
- nucleic acid
- high resolution
- stem cells
- peripheral blood
- dendritic cells
- cell death
- cell proliferation
- acute lymphoblastic leukemia
- endoplasmic reticulum stress