Chondroprotective Effects of Chondrogenic Differentiated Adipose-Derived Mesenchymal Stem Cells Sheet on Degenerated Articular Cartilage in an Experimental Rabbit Model.
Atsushi TaninakaTamon KabataKatsuhiro HayashiYoshitomo KajinoDaisuke InoueTakaaki OhmoriKen UeokaYuki YamamuroTomoyuki KataokaYoshitomo SaikiYu YanagiMusashi ImaTakahiro IyobeHiroyuki TsuchiyaPublished in: Bioengineering (Basel, Switzerland) (2023)
Adipose-derived stem cells (ADSCs) have been studied for many years as a therapeutic option for osteoarthritis (OA); however, their efficacy remains insufficient. Since platelet-rich plasma (PRP) induces chondrogenic differentiation in ADSCs and the formation of a sheet structure by ascorbic acid can increase the number of viable cells, we hypothesized that the injection of chondrogenic cell sheets combined with the effects of PRP and ascorbic acid may hinder the progression of OA. The effects of induction of differentiation by PRP and formation of sheet structure by ascorbic acid on changes in chondrocyte markers (collagen II, aggrecan, Sox9) in ADSCs were evaluated. Changes in mucopolysaccharide and VEGF-A secretion from cells injected intra-articularly in a rabbit OA model were also evaluated. ADSCs treated by PRP strongly chondrocyte markers, including type II collagen, Sox9, and aggrecan, and their gene expression was maintained even after sheet-like structure formation induced by ascorbic acid. In this rabbit OA model study, the inhibition of OA progression by intra-articular injection was improved by inducing chondrocyte differentiation with PRP and sheet structure formation with ascorbic acid in ADSCs.
Keyphrases
- platelet rich plasma
- knee osteoarthritis
- gene expression
- induced apoptosis
- mesenchymal stem cells
- stem cells
- cell cycle arrest
- transcription factor
- dna methylation
- wound healing
- ultrasound guided
- single cell
- signaling pathway
- type diabetes
- vascular endothelial growth factor
- cell death
- metabolic syndrome
- insulin resistance