Circulating Platelet-Derived Extracellular Vesicles Are a Hallmark of Sars-Cov-2 Infection.
Annalisa ChiocchettiDavide RaineriRoberta RollaMara GiordanoChiara PuricelliBeatrice VilardoMarcello ManfrediVincenzo CantaluppiPier Paolo SainaghiLuigi Mario CastelloNello De VitaLorenza ScottiRosanna VaschettoUmberto DianzaniAnnalisa ChiocchettiPublished in: Cells (2021)
Sars-Cov-2 infection causes fever and cough that may rapidly lead to acute respiratory distress syndrome (ARDS). Few biomarkers have been identified but, unfortunately, these are individually poorly specific, and novel biomarkers are needed to better predict patient outcome. The aim of this study was to evaluate the diagnostic performance of circulating platelets (PLT)-derived extracellular vesicles (EVs) as biomarkers for Sars-Cov-2 infection, by setting a rapid and reliable test on unmanipulated blood samples. PLT-EVs were quantified by flow cytometry on two independent cohorts of Sars-CoV-2+ (n = 69), Sars-Cov-2- (n = 62) hospitalized patients, and healthy controls. Diagnostic performance of PLT-EVs was evaluated by receiver operating characteristic (ROC) curve. PLT-EVs count were higher in Sars-Cov-2+ compared to Sars-Cov-2- patients or HC. ROC analysis of the combined cohorts showed an AUC = 0.79 and an optimal cut-off value of 1472 EVs/μL, with 75% sensitivity and 74% specificity. These data suggest that PLT-EVs might be an interesting biomarker deserving further investigations to test their predictive power.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- acute respiratory distress syndrome
- extracorporeal membrane oxygenation
- flow cytometry
- mechanical ventilation
- end stage renal disease
- coronavirus disease
- chronic kidney disease
- newly diagnosed
- ejection fraction
- electronic health record
- peritoneal dialysis
- big data
- prognostic factors
- intensive care unit
- patient reported outcomes
- peripheral blood
- patient reported