Printed silk microelectrode arrays for electrophysiological recoding and controlled drug delivery.
Nouran AdlyTetsuhiko F TeshimaHossein HassaniGeorge Al BoustaniLennart K WeißGordon ChengJoe AlexanderBernhard WolfrumPublished in: Advanced healthcare materials (2023)
The use of soft and flexible bioelectronic interfaces can enhance the quality for recording cells' electrical activity by ensuring a continuous and intimate contact with the smooth, curving surfaces found in the physiological environment. This work develops soft microelectrode arrays (MEAs) made of silk fibroin (SF) films for recording interfaces that can also serve as a drug delivery system. We use inkjet printing as a tool to deposit the substrate, conductive electrode, and insulator, as well as a drug-delivery nanocomposite film. This approach is highly versatile, as shown in the fabrication of carbon microelectrodes, sandwiched between a silk substrate and a silk insulator. The technique permits the development of thin-film devices that can be employed for in vitro extracellular recordings of HL-1 cell action potentials. We also demonstrate the tuning of SF by applying an electrical stimulus to produce a permeable layer that can be used in on-demand drug delivery systems. The multifunctional MEA developed here can pave the way for in vitro drug screening by applying time-resolved and localized chemical stimuli. This article is protected by copyright. All rights reserved.
Keyphrases
- tissue engineering
- drug delivery
- cancer therapy
- reduced graphene oxide
- wound healing
- induced apoptosis
- carbon nanotubes
- room temperature
- single cell
- cell cycle arrest
- drug release
- high density
- gold nanoparticles
- cystic fibrosis
- pseudomonas aeruginosa
- quantum dots
- emergency department
- signaling pathway
- solid state
- low cost
- biofilm formation
- mesenchymal stem cells
- highly efficient
- cell proliferation
- amino acid
- drug induced
- metal organic framework
- solid phase extraction