Application of the 3' mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue.

Jin Sung JangEileen HolickyJulie LauSamantha McDonoughMark MutaweMatthew J KosterKenneth J WarringtonJulie M Cuninngham

Published in: BMC genomics (2021)

In this study, QuantSeq 3' mRNA-Seq with PCR bias correction using UMI is shown to be a suitable method for gene quantification in both FF and FFPE RNAs. 3' mRNA-Seq with UMI may be applied to severely degraded RNA from FFPE tissues generating high-quality sequencing data.

Keyphrases

genome wide
single cell
rna seq
dna methylation
binding protein
copy number
gene expression
nucleic acid
big data
machine learning
single molecule
data analysis
artificial intelligence