De-novo identification of specific exposure biomarkers of the alternative plasticizer di(2-ethylhexyl) terephthalate (DEHTP) after low oral dosage to male volunteers by HPLC-Q-Orbitrap-MS.
Frederik LessmannDaniel BuryTobias WeissHeiko HayenThomas BrüningHolger M KochPublished in: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals (2018)
The excretion profiles obtained by HPLC-Q-Orbitrap-MS were in good agreement with quantitative HPLC-QqQ-MS data. For the newly discovered metabolites, plausible excretion profiles, similar to the ones of the known metabolites, were obtained. The presented approach proved to be successful for metabolite screening in urine samples after low-dose exposure and will be applied in future human metabolism studies for a fast, reliable and cost effective identification of specific biomarkers of exposure.
Keyphrases
- ms ms
- mass spectrometry
- ultra high performance liquid chromatography
- low dose
- high resolution
- liquid chromatography
- high performance liquid chromatography
- tandem mass spectrometry
- simultaneous determination
- endothelial cells
- high resolution mass spectrometry
- multiple sclerosis
- gas chromatography
- big data
- solid phase extraction
- electronic health record
- bioinformatics analysis
- cystic fibrosis
- deep learning
- candida albicans