Genetic and Phenotypic Characterization of the First Canadian Case of Ambler Class A Carbapenemase FRI-8.
Laura F MatasejeFlorence Doualla-BellDavid A BoydKen FakharuddinHector Felipe Garcia JeldesValerie PlanteMarie-Claude BeaudoinAlexandre GodboutSimon WongJudith FafardMichael R MulveyPublished in: Microbial drug resistance (Larchmont, N.Y.) (2023)
This study investigated the mechanism of carbapenem resistance in an Enterobacter cloacae complex positive by the modified carbapenem inactivation method (mCIM) but negative by the Rosco Neo-Rapid Carb Kit, β CARBA, and conventional PCR for common carbapenemase genes (KPC, NDM, OXA-48, IMP, VIM, GES, and IMI/NMC). Using whole genome sequencing (WGS) data we confirmed the identification of Enterobacter asburiae (ST1639) and the presence of bla FRI-8 located on a 148kb IncFII(Yp) plasmid. This is the first occurrence of a clinical isolate harboring the FRI-8 carbapenemase and the second occurrence of FRI in Canada. This study highlights the need to use both WGS and phenotypic screening methods for detection of carbapenemase-producing strains if we consider the growing diversity of carbapenemases.
Keyphrases
- klebsiella pneumoniae
- escherichia coli
- multidrug resistant
- acinetobacter baumannii
- gram negative
- genome wide
- risk assessment
- drug resistant
- gene expression
- electronic health record
- crispr cas
- dna methylation
- pseudomonas aeruginosa
- transcription factor
- cystic fibrosis
- quantum dots
- single molecule
- bioinformatics analysis
- label free