Human Papillomavirus DNA Detection by Droplet Digital PCR in Formalin-Fixed Paraffin-Embedded Tumor Tissue from Oropharyngeal Squamous Cell Carcinoma Patients.
Camila Marques SchiavettoPriscila Marinho de AbreuSandra Ventorin von ZeidlerLaís Machado de JesusRaiany Santos CarvalhoMaria Thereza CirinoAdriana Cruvinel CarloniCristina OliveiraCristovam Scapulatempo-NetoGisele Caravina de AlmeidaNei Soares de MenezesAndré Lopes CarvalhoRui Manuel ReisAna Carolina de CarvalhoPublished in: Molecular diagnosis & therapy (2020)
Type-specific HPV-DNA detection by ddPCR is highly specific but moderately sensitive for the determination of HPV status and showed clinical relevance, mainly when associated with p16-IHC status. Results highlight the importance of performing HPV-DNA testing in combination with p16-IHC for proper identification of HPV-associated OPSCC and to improve clinical management of OPSCC patients.