Simultaneous recognition of dopamine and uric acid in real samples through highly sensitive new electrode fabricated using ZnO/carbon quantum dots: bio-imaging and theoretical studies.
Eduardo D Tecuapa-FloresCristian B Palacios-CabreraAlan J Santiago-CuevasJosé G HernándezJayanthi NarayananThangarasu PandiyanPublished in: The Analyst (2023)
Dopamine (DA) and uric acid (UA), which are vital components in human metabolism, cause several health problems if they are present in altered concentrations; thus, the determination of DA and UA is essential in real samples using selective sensors. In the present study, graphite carbon paste electrodes (CPE) were fabricated using ZnO/carbon quantum dots (ZnO/CQDs) and employed as electrochemical sensors for the detection of DA and UA. These electrodes were fully characterized via different analytical techniques (XRD, SEM, TEM, XPS, and EDS). The electrochemical responses from the modified electrodes were evaluated using cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy. The results showed that the present electrode has exhibited high sensitivity towards DA, recognizing even at low concentrations (0.12 μM), and no inference was observed in the presence of UA. The ZnO/CQD electrode was applied for the simultaneous detection of co-existing DA and UA in real human urine samples and the peak potential separation between DA and UA was found to be greatly associated with the synergistic effect originated from ZnO and CQDs. The limit of detection (LOD) of the electrode was analyzed, and compared with other commercially available electrodes. Thus, the ZnO/CQD electrode was used to detect DA and UA in real samples, such as Saccharomyces cerevisiae cells.
Keyphrases
- uric acid
- quantum dots
- reduced graphene oxide
- carbon nanotubes
- solid state
- label free
- gold nanoparticles
- metabolic syndrome
- room temperature
- molecularly imprinted
- sensitive detection
- endothelial cells
- loop mediated isothermal amplification
- saccharomyces cerevisiae
- ionic liquid
- mental health
- visible light
- high resolution
- healthcare
- energy transfer
- induced apoptosis
- real time pcr
- computed tomography
- magnetic resonance
- light emitting
- living cells
- climate change
- single cell
- liquid chromatography
- cell cycle arrest
- endoplasmic reticulum stress
- signaling pathway
- simultaneous determination