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Reversible Photoisomerization of the Isolated Green Fluorescent Protein Chromophore.

Eduardo CarrascosaJames N BullMichael S ScholzNeville J A CoughlanSeth OlsenUta WilleEvan J Bieske
Published in: The journal of physical chemistry letters (2018)
Fluorescent proteins have revolutionized the visualization of biological processes, prompting efforts to understand and control their intrinsic photophysics. Here we investigate the photoisomerization of deprotonated p-hydroxybenzylidene-2,3-dimethylimidazolinone anion (HBDI-), the chromophore in green fluorescent protein and in Dronpa protein, where it plays a role in switching between fluorescent and nonfluorescent states. In the present work, isolated HBDI- molecules are switched between the Z and E forms in the gas phase in a tandem ion mobility mass spectrometer outfitted for selecting the initial and final isomers. Excitation of the S1 ← S0 transition provokes both Z → E and E → Z photoisomerization, with a maximum response for both processes at 480 nm. Photodetachment is a minor channel at low light intensity. At higher light intensities, absorption of several photons in the drift region drives photofragmentation, through channels involving CH3 loss and concerted CO and CH3CN loss, although isomerization remains the dominant process.
Keyphrases
  • quantum dots
  • living cells
  • protein protein
  • label free
  • amino acid
  • high resolution
  • binding protein
  • fluorescent probe
  • high intensity
  • ionic liquid
  • small molecule
  • mass spectrometry