OH radical reactions with the hydrophilic component of sphingolipids.
Alexandra G LisovskayaOleg ShadyroOlav SchiemannIan CarmichaelPublished in: Physical chemistry chemical physics : PCCP (2021)
In this work, using the example of model compounds, we studied the reactions resulting from the interaction of OH radicals with the hydrophilic part of sphingolipids. We compared the stopped-flow EPR spectroscopy and pulse radiolysis with optical detection methods to characterize radical intermediates formed in the reaction of OH radicals with glycerol, serinol and N-boc-serinol. Quantum chemical calculations were also performed to help interpret the observed experimental data. It was shown that H-abstraction from the terminal carbon atom is the main process that is realized for all the studied compounds. The presence of the unsubstituted amino group (-NH2) is seen to completely change the reaction properties of serinol in comparison with those observed in glycerol and N-boc serinol.
Keyphrases
- molecular dynamics
- liquid chromatography
- high resolution
- solid state
- density functional theory
- electron transfer
- blood pressure
- electronic health record
- mass spectrometry
- monte carlo
- solid phase extraction
- label free
- single molecule
- loop mediated isothermal amplification
- molecular dynamics simulations
- big data
- room temperature
- high speed
- quantum dots