Ternary recognition fluorescent probe for lysosome acidification counter-ion studies via Cl - , K + , and pH.

Lysosomal acidity relies on H + inflow, which requires counter-ion flows (Cl - and K + ) to balance charge. A lysosome targeting ternary recognition fluorescent probe for Cl - , K + , and pH was developed for lysosome acidification counter-ion research. The probe was used to study counter-ion changes when the Cl - channel was blocked and under oxidative pressure.