Avocado Oil Extract Modulates Auditory Hair Cell Function through the Regulation of Amino Acid Biosynthesis Genes.
Youn Hee NamIsabel RodriguezSeo Yeon JeongThu Nguyen Minh PhamWanlapa NuankaewYun Hee KimRodrigo CastañedaSeo Yule JeongMin Seon ParkKye Wan LeeJung Suk LeeDo Hoon KimYu Hwa ParkSeung Hyun KimIn Seok MoonSe-Young ChoungBin Na HongKwang Won JeongTong Ho KangPublished in: Nutrients (2019)
Sensorineural hearing loss (SNHL) is one of the most common causes of disability, affecting over 466 million people worldwide. However, prevention or therapy of SNHL has not been widely studied. Avocado oil has shown many health benefits but it has not yet been studied in regards to SNHL. Therefore, we aimed to investigate the efficacy of avocado oil on SNHL in vitro and in vivo and elucidate its mode of action. For the present study, we used enhanced functional avocado oil extract (DKB122). DKB122 led to recovery of otic hair cells in zebrafish after neomycin-induced otic cell damage. Also, DKB122 improved auditory sensory transmission function in a mouse model of noise induced-hearing loss and protected sensory hair cells in the cochlea. In addition, RNA sequencing was performed to elucidate the mechanism involved. KEGG pathway enrichment analysis of differentially expressed genes showed that DKB122 protected House Ear Institute-Organ of Corti 1 (HEI-OC1) cells against neomycin-related alterations in gene expression due to oxidative stress, cytokine production and protein synthesis.
Keyphrases
- induced apoptosis
- oxidative stress
- diabetic rats
- gene expression
- cell cycle arrest
- hearing loss
- mouse model
- single cell
- endoplasmic reticulum stress
- amino acid
- dna methylation
- healthcare
- signaling pathway
- public health
- genome wide
- working memory
- dna damage
- high glucose
- mental health
- cell death
- mesenchymal stem cells
- transcription factor
- bioinformatics analysis
- stress induced
- bone marrow
- health information
- smoking cessation
- chemotherapy induced