Exploring the complex map of insulin polymorphism: a novel crystalline form in the presence of m-cresol.
Fotini KaravassiliAlexandros ValmasMaria DimarogonaAnastasia E GiannopoulouStavroula FiliMathias NorrmanGerd SchluckebierDetlef BeckersAndrew N FitchIrene MargiolakiPublished in: Acta crystallographica. Section D, Structural biology (2020)
In this study, the first crystal structure of a novel crystal form of human insulin bound to meta-cresol in an acidic environment is reported. The combination of single-crystal and powder X-ray diffraction crystallography led to the detection of a previously unknown monoclinic phase (P21). The structure was identified from the powder patterns and was solved using single-crystal diffraction data at 2.2 Å resolution. The unit-cell parameters at pH 6.1 are a = 47.66, b = 70.36, c = 84.75 Å, β = 105.21°. The structure consists of two insulin hexamers per asymmetric unit. The potential use of this insulin form in microcrystalline drugs is discussed.
Keyphrases
- type diabetes
- glycemic control
- endothelial cells
- solid state
- single cell
- high resolution
- magnetic resonance imaging
- computed tomography
- single molecule
- risk assessment
- electron microscopy
- mesenchymal stem cells
- room temperature
- skeletal muscle
- bone marrow
- adipose tissue
- mass spectrometry
- drug induced
- climate change
- human health