Profiling of polar ionogenic metabolites in Polish wines by capillary electrophoresis-mass spectrometry.
Marlien van MeverMagdalena FabjanowiczMaricruz Mamani-HuancaÁngeles López-GonzálvezJustyna Płotka-WasylkaRawi RamautarPublished in: Electrophoresis (2022)
The composition of wine is determined by a complex interaction between environmental factors, genetic factors (i.e., grape varieties), and winemaking practices (including technology and storage). Metabolomics using NMR spectroscopy, GC-MS, and/or LC-MS has shown to be a useful approach for assessing the origin, authenticity, and quality of various wines. Nonetheless, the use of additional analytical techniques with complementary separation mechanisms may aid in the deeper understanding of wine's metabolic processes. In this study, we demonstrate that CE-MS is a very suitable approach for the efficient profiling of polar ionogenic metabolites in wines. Without using any sample preparation or derivatization, wine was analyzed using a 10-min CE-MS workflow with interday RSD values for 31 polar and charged metabolites below 3.8% and 23% for migration times and peak areas, respectively. The utility of this workflow for the global profiling of polar ionogenic metabolites in wine was evaluated by analyzing different cool-climate Polish wine samples.
Keyphrases
- copy number
- mass spectrometry
- ms ms
- liquid chromatography
- capillary electrophoresis
- high performance liquid chromatography
- high resolution mass spectrometry
- gas chromatography
- tandem mass spectrometry
- dna methylation
- single cell
- ionic liquid
- high resolution
- liquid chromatography tandem mass spectrometry
- primary care
- ultra high performance liquid chromatography
- simultaneous determination
- healthcare
- climate change
- electronic health record
- solid phase extraction
- gas chromatography mass spectrometry
- energy transfer
- molecularly imprinted
- gene expression