Genetic analysis and QTL mapping for silique density in rapeseed (Brassica napus L.).
Xingrong MaJinhua WangYongfen GuPengpeng FangWenjing NieRuirui LuoJin LiuWei QianJiaqin MeiPublished in: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik (2023)
by using a genetic linkage map constructed by restriction site-associated DNA sequencing (RAD seq) technology. A total of eight, 14 and three QTLs were identified for SDMI, SNMI and MIL under three environments, respectively, with an overlap among SDMI and SNMI in 55.7-75.4 cm on linkage group C06 which corresponding to 11.6-27.3 Mb on chromosome C06. Genomic resequencing was further conducted between a high- and a low-SDMI pool constructed from the DH population, and QTL-seq analysis identified a 0.15 Mb interval (25.98-26.13 Mb) from the C06-QTL region aforementioned. Transcriptome sequencing and qRT-PCR identified one possible candidate gene (BnARGOS) from the 0.15 Mb interval. This study will provide novel insights into the genetic basis of SD in rapeseed.
Keyphrases
- genome wide
- copy number
- high density
- single cell
- dna methylation
- rna seq
- wastewater treatment
- dna damage
- high resolution
- oxidative stress
- circulating tumor
- cell free
- single molecule
- gene expression
- solid phase extraction
- antiretroviral therapy
- metal organic framework
- transcription factor
- hiv infected
- hiv testing
- genome wide analysis