Chemopreventive effect and induction of DNA repair by oenothein B ellagitannin isolated from leaves of Eugenia uniflora in Swiss Webster treated mice.
Cinthia Aparecida SilvaJefferson Hollanda VérasJoyce Aves VenturaAbel Vieira de Melo BisnetoMatheus Gabriel de OliveiraElisa Flávia Luiz Cardoso BailãoCarolina Ribeiro E SilvaCléver Gomes CardosoSuzana Costa SantosLee Chen-ChenPublished in: Journal of toxicology and environmental health. Part A (2023)
Oenothein B (OeB) is a dimeric ellagitannin with potent antioxidative, antitumor, immunomodulatory, and anti-inflammatory properties. Despite the promising activities of OeB, studies examining the genotoxic or protective effects of this ellagitannin on DNA are scarce. Therefore, to further comprehensively elucidate the chemopreventive profile of OeB, the aim of this study was to evaluate the mutagenic and antimutagenic actions of OeB using Salmonella typhimurium strains with the Ames test. The micronucleus (MN) test and comet assay were used to assess the anticytotoxic and antigenotoxic effects of OeB on mouse bone marrow cells following differing treatments (pre-, co-, and post-treatment) in response to cyclophosphamide (CPA)-induced DNA damage. In addition, histopathological analyses were performed to assess liver and kidney tissues of Swiss Webster treated mice. Our results did not detect mutagenic or antimutagenic activity attributed to OeB at any concentration in the Ames test. Regarding the MN test, data showed that this ellagitannin exerted antigenotoxic and anticytotoxic effects against CPA-induced DNA damage under all treatment conditions. However, no anticytotoxic action was observed in MN test after pre-treatment with the highest doses of OeB. In addition, OeB demonstrated antigenotoxic effects in the comet assay for all treatments. Histopathological analyses indicated that OeB attenuated the toxic effects of CPA in mouse liver and kidneys. These findings suggest that OeB exerted a chemoprotective effect following pre- and co-treatments and a DNA repair action in post-treatment experiments. Our findings indicate that OeB protects DNA against CPA-induced damaging agents and induces post-damage DNA repair.
Keyphrases
- dna repair
- dna damage
- oxidative stress
- bone marrow
- dna damage response
- anti inflammatory
- type diabetes
- low dose
- gene expression
- mesenchymal stem cells
- single molecule
- machine learning
- combination therapy
- induced apoptosis
- metabolic syndrome
- artificial intelligence
- high throughput
- cell free
- skeletal muscle
- high dose
- big data
- circulating tumor cells
- single cell
- transition metal